Structural Glycobiology Team

Team Leader

Yoshiki Yamaguchi

  • D.Pharm.
  • Yoshiki Yamaguchi
  • Brief resume
    1998
    D.Pharm., The University of Tokyo
    1998
    Research Fellow, Japan Society for the Promotion of Science
    2000
    Research Assistant, The University of Tokyo
    2001
    Lecturer, Nagoya City University
    2007
    Team Leader, Structural Glycobiology Team, RIKEN (-current)
    2007
    Visiting Professor, Nagoya City University (-current)
    2008
    Visiting Professor, Tokyo Medical and Dental University (-current)

Outline

Structural Glycobiology Team

In order to elucidate the functions of the glycans attached to proteins, it is important to analyze the glycans from the structural point of view. Our team aims to reveal the functions of glycans through the three-dimensional structures of glycoproteins and oligosaccharide-related proteins. We prepare sample glycoconjugates with organic synthesis and cell-engineering techniques, and determine their three-dimensional structures using biophysical methods such as NMR or X-ray crystallography. Furthermore, we construct databases of oligosaccharide structures using bioinformatics-based approaches and decode the “glycosignals” from the structural information. We particularly focus on disease-related glycoproteins, and develop diagnostic and therapeutic agents.

Recent Research Topic

Construction and analysis of glycan conformation database
Fig. 1: Construction and analysis of glycan conformation database

Selected Publications

  1. M. Kanagawa, T. Satoh, A. Ikeda, Y. Nakano, H. Yagi, K. Kato, K. Kojima-Aikawa, Y. Yamaguchi, Crystal structures of human secretory proteins ZG16p and ZG16b reveal a Jacalin-related β-prism fold, Biochem. Biophys. Res. Commun. 2011, 404, 201.
  2. T. Satoh, Y. Chen, D. Hu, S. Hanashima, K. Yamamoto, Y. Yamaguchi, Structural basis for oligosaccharide recognition of misfolded glycoproteins by OS-9 in ER-associated degradation, Molecular Cell 2010, 40, 905.
  3. Y. Yamaguchi, K. Kato, Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy, Methods Enzymol. 2010, 478, 305.
  4. K. Kato, Y. Yamaguchi, Y. Arata, Stable-isotope-assisted NMR approaches to glycoproteins using immunoglobulin G as a model system, Prog. NMR Spect. 2010, 56, 346.
  5. S. Hanashima, K. Sato, Y. Naito, H. Takematsu, Y. Kozutsumi, Y. Ito, Yamaguchi Y, Synthesis and binding analysis of unique AG2 pentasaccharide to human Siglec-2 using NMR techniques, Bioorg. Med. Chem. 2010, 18, 3720.
  6. S. Sato, O. Morohara, D. Fujita, Y. Yamaguchi, K. Kato, M. Fujita, Parallel-stacked aromatic hosts for orienting small molecules in a magnetic field: induced residual dipolar coupling by encapsulation, J. Am. Chem. Soc. 2010, 132, 3670.
  7. M. Fujita, Y. Maeda, M. Ra, Y. Yamaguchi, R. Taguchi, T. Kinoshita, GPI glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER to the Golgi, Cell 2009, 139, 352.
  8. S. Hanashima, K. Sato, Y. Ito, Y. Yamaguchi, Silylene/oxazolidinone double-locked sialic acid building blocks for the efficient sialylation reaction in dichloromethane, Eur. J. Org. Chem. 2009, 4215.
  9. S. Hanashima, K. Sato, Y. Ito, Y. Yamaguchi, Synthesis of the Starfish Ganglioside AG2 Pentasaccharide, Tetrahedron Lett. 2009, 50, 6150.
  10. Y. Yamaguchi, M. Wälchli, M. Nagano, K. Kato, A 13C-detection NMR approach for large glycoproteins, Carbohydr. Res. 2009, 344, 535.

Core Members

Principal Investigator add delete
Yoshiki Yamaguchi Team Leader    
Staff Scientist add delete
Shinya Hanashima ASI Research Scientist    
Mayumi Kanagawa ASI Research Scientist    
Masamichi Nagae ASI Research Scientist    
Postdoctoral Fellow add delete
Student Trainee add delete
Ganesh Prasad Subedi International Program Associate    
Technical Assistant add delete
Masaki Kato Technical Staff I    
Akemi Ikeda Technical Staff I    
Akitsugu Suga Technical Staff I    
Administrative Assistant add delete
Visiting Research Staff add delete
Yutaro Hayashi Senior Visiting Scientist    
Other Staff add delete
Jun Uzawa Part-timer1    
Noriko Tanaka Part-timer2    
Kana Matsumoto Agency Staff    
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