Glycometabolome Team

Team Leader

Tadashi Suzuki

  • D.Sci.
  • Tadashi Suzuki
  • Brief resume
    1997
    D.Sci., University of Tokyo
    1997
    Postdoctoral Fellow/Research Scientist/Research Assistant Professor, State University of New York at Stony Brook, USA
    2001
    Researcher, PRESTO, Japan Science and Technology Corporation
    2002
    Assistant Professor, University of Tokyo
    2004
    Visiting Associate Professor, Osaka University
    2007
    Team Leader, Glycometabolome Team, RIKEN (-current)
    2010
    Visiting Professor, Graduate School of Science and Engineering, Saitama University (-current)

Outline

Glycometabolome Team

Peptide:N-glycanase (PNGase) releases N-glycans from glycoproteins. The cytoplasmic PNGases, ubiquitously found throughout eukaryotes, are now widely recognized as a component implicated in the ERAD (ER-associated degradation) process, which constitutes one of the quality control machines for newly synthesized misfolded glycoproteins exported out of the ER lumen. The catabolic pathway for the “free” N-glycans released by the PNGase in the cytosol remains largely unknown. Although this “non-lysosomal” metabolic path for N-glycan may represent one of the very basic biological phenomena in eukaryotes, there are still many more enzymes/transporters that remain to be identified. We are currently trying to identify other players involved in this process, and examining a number of approaches to analyze the physiological importance of this non-lysosomal metabolic pathway.

Recent Research Topic

Proposed non-lysosomal catabolic pathway for N-glycans in mammalian cells
Fig. 1: Proposed non-lysosomal catabolic pathway for N-glycans in mammalian cells
There are many processes (indicated with question marks) still left unidentified.
Fig. 1
Reproduced, with permission, from T. Suzuki, Cytoplasmic peptide:N-glycanase and catabolic pathway for free N-glycans in the cytosol, Seminars Cell Dev. Biol. 2007, 18, 762. © (2012) Elsevier

Selected Publications

  1. Y. Haga, K. Ishii, T. Suzuki, N-Glycosylation is critical for the stability and intracellular trafficking of glucose transporter GLUT4, J. Biol. Chem. 2011, 286, 31320.
  2. A. Kato∗, L. Wang∗, K. Ishii, J. Seino, N. Asano, T. Suzuki, Calystegine B3 as a specific inhibitor for cytoplasmic α-mannosidase, Man2C1, J. Biochem. 2011, 149, 415. (∗: equally contributed)
  3. A. Hosomi, K. Tanabe, H. Hirayama, I., Kim, H. Rao, T. Suzuki, Identification of Htm1(EDEM)-dependent, Mns1-independent ERAD pathway in Saccharomyces cerevisiae. Application of a novel assay for glycoprotein ERAD, J. Biol. Chem. 2010, 285, 24324.
  4. Y. Funakoshi, Y. Negishi, J. P. Gergen, J. Seino, K. Ishii, W. J. Lennarz, I. Matsuo, Y. Ito, N. Taniguchi, T. Suzuki, Evidence for an essential deglycosylation-independent activity of PNGase in Drosophila melanogaster, PLoS One, 2010, 5, e10545.
  5. H. Hirayama, J. Seino, T. Kitajima, Y. Jigami, T. Suzuki, Free oligosaccharides to monitor glycoprotein endoplasmic reticulum-associated degradation in Saccharomyces cerevisiae, J. Biol. Chem. 2010, 285, 12390.
  6. S. Maerz, Y. Funakoshi, Y. Negishi, T. Suzuki, S. Seiler, The Neurospora peptide:N-glycanase ortholog PNG1 is essential for cell polarity despite its lack of enzymatic activity, J. Biol. Chem. 2010, 285, 2326.
  7. Y. Haga, K. Totani, Y. Ito, T. Suzuki, Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol, Glycobiology 2009, 19, 987.
  8. Y. Funakoshi, T. Suzuki, Glycobiology in the cytosol: the bitter side of a sweet world, Biochim. Biophys. Acta 2009, 1790, 81.
  9. T. Suzuki, I. Matsuo, K. Totani, S. Funayama, J. Seino, N. Taniguchi, Y. Ito, S. Hase, Dual-gradient HPLC for identification of cytosolic high mannose-type free glycans, Anal. Biochem. 2008, 381, 224.
  10. A. Ishizuka, Y. Hashimoto, R. Naka, M. Kinoshita, K. Kakehi#, J. Seino, Y. Funakoshi, T. Suzuki#, A. Kameyama, H. Narimatsu, Accumulation of free complex-type N-glycans in stomach cancer cells, MKN7 and MKN45. Biochem. J. 2008, 413, 227. (#-corresponding authors)
  11. T. Suzuki, Cytoplasmic peptide:N-glycanase and catabolic pathway for free N-glycans in the cytosol, Sem. Cell Develop. Biol. 2007, 18, 762.

Core Members

Principal Investigator add delete
Tadashi Suzuki Team Leader    
Staff Scientist add delete
Postdoctoral Fellow add delete
Akira Hosomi Postdoctoral Researcher    
Hiroto Hirayama Postdoctoral Researcher    
Li Wang Postdoctoral Researcher    
Yoichiro Harada Postdoctoral Researcher    
Yoshimi Haga Visiting Researcher    
Student Trainee add delete
Chengcheng Huang International Program Associate    
Technical Assistant add delete
Junichi Seino Technical Staff I    
Yuki Negishi Technical Staff I    
Kumiko Ishii Technical Staff II    
Administrative Assistant add delete
Visiting Research Staff add delete
Other Staff add delete
Kotoko Ueno Part-timer2    
Yae Tsuchiya Part-timer2    
Tsugiyo Matsuda Part-timer2    
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