Chemical Library Validation Team

Team Head

Nobumoto Watanabe

  • D.Sci.
  • Nobumoto Watanabe
  • Brief resume
    1987
    D.Sci., University of Tokyo
    1987
    Postdoctoral Researcher, Molecular Oncology Laboratory, RIKEN
    1989
    Research Scientist, Gene Bank, RIKEN
    1992
    Research Associate, Salk Institute, USA
    2001
    Senior Research Scientist, Antibiotics Laboratory, RIKEN
    2006
    Visiting Professor, Universiti Sains Malaysia: USM, Malaysia (-current)
    2008
    Senior Research Scientist, Chemical Library Validation Team, RIKEN
    2009
    Team Head, Chemical Library Validation Team, RIKEN (-current)

Outline

Chemical Library Validation Team

The Chemical Biology Core Facility collects many natural compounds and stores them in the RIKEN Natural Product Depository (NPDepo). A good chemical library should contain many compounds with a wide range of useful biological activities; therefore our team validates the activities of the compounds in NPDepo by actual screening of the library through various assay systems. For this purpose, we develop high throughput screening systems using chemical array and liquid handling robots to screen the large library. Besides developing new screening systems to identify compounds with novel biological activities, our team also conducts basic research in to the mechanisms behind the biological activities of the compounds.

Recent Research Topic

Examples of Chemical library Validation Systems
Fig. 1: Examples of Chemical library Validation Systems

Selected Publications

  1. E. B. B. Ong, et al. Vipirinin, a coumarin-based HIV-1 Vpr Inhibitor, interacts with a hydrophobic region of Vpr, J. Biol. Chem. 2011, 286, 14049.
  2. M. Kawatani, et al. Identification of a small-molecule inhibitor of DNA topoisomerase II by proteomic profiling, Chem. Biol. 2011, 18, 743.
  3. I. Miyazaki, S. Simizu, H. Okumura, S. Takagi, H. Osada, A small molecule inhibitor shows that pirin regulates migration of melanoma cell, Nature Chem. Biol. 2010, 6, 667.
  4. M. Muroi, et al. Application of proteomic profiling based on 2D-DIGE for classification of compounds according to the mechanism of action, Chem. Biol. 2010, 17, 460.
  5. N. Watanabe, et al. Deficiency in chromosome congression by the inhibition of PLK1 polo box domain-dependent recognition, J. Biol. Chem. 2009, 284, 2344.
  6. Y. Tamura, et al. Polo-like kinase 1 phosphorylates and regulates Bcl-xL during pironetin-induced apoptosis, Oncogene 2009, 28, 107.
  7. M. Kawatani, et al. The identification of an osteoclastogenesis inhibitor through the inhibition of glyoxalase I, Proc. Natl Acad. Sci. USA 2008, 105, 11691.

Core Members

Principal Investigator add delete
Nobumoto Watanabe Team Head    
Staff Scientist add delete
Tatsuhiko Sudo Senior Research Scientist    
Makoto Muroi Senior Research Scientist    
Takuya Ito Senior Research Scientist    
Yasumitsu Kondoh Senior Research Scientist    
Akira Wada Senior Research Scientist    
Makoto Kawatani Research Scientist    
Postdoctoral Fellow add delete
Hiroki Hayase Postdoctoral Researcher    
Tatsuro Kawamura Postdoctoral Researcher    
Alena Lagumdzija Visiting Researcher    
Student Trainee add delete
Technical Assistant add delete
Hideaki Konno Technical Staff I    
Kaori Honda Technical Staff I    
Hisae Kondo Technical Staff I    
Yasuko Hirata Technical Staff I    
Harumi Aono Technical Staff I    
Emiko Sanada Technical Staff I    
Yukako Fukushima Technical Staff I    
Shinobu Yamaguchi Technical Staff I    
Tomomi Sekine Technical Staff I    
Administrative Assistant add delete
Visiting Research Staff add delete
Osong Kwon Visiting Scientist    
Other Staff add delete
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